Isolation and characterization of microsatellite loci in the Charal de Xochimilco Chirostoma humboldtianum

Abstract

Microsatellite loci were isolated and characterized for the endemic fish Chirostoma humboldtianum usingan enrichment procedure. Eight polymorphic microsatellites were genotyped for 32 - 48 individuals from TepuxtepecDam, Michoacán. The number of alleles per locus ranged from 3 to 11 and the average observed and expectedheterozygosities were 0.61 and 0.63, respectively. All loci deviated significantly from Hardy-Weinberg expectations,which might be related to small population sizes associated to human disturbances and habitat loss. These are thefirst loci described for the species and the genus and could be useful in studies of population genetics, conservationand management of the species.