In vitro propagation and adaptation of plants to ex vitro conditions of Kroenleinia grusonii (Cactaceae)
DOI:
https://doi.org/10.22201/ib.20078706e.2026.97.5700Keywords:
Indole-3-acetic acid, 6-bencylaminoourine, Shoots, Cactus, Endemic, RootingAbstract
Kroenleinia grusonii (Hildm.) Lodé, is a cactus endemic to central Mexico, that faces risks such as illegal collection or modification of its natural habitat and is listed as endangered. The objective of this work was to evaluate different concentrations of 6-benzylaminopurine (BAP) alone or combined with indoleacetic acid (IAA) in K. grusonii and to induce root formation to assess plant adaptation to ex vitro conditions. Shoot induction was with BAP (0, 1.0, 2.0 and 3.0 mg/L), IAA (0.5 mg/L) and BAP (0, 1.0, 2.0 and 3.0 mg/L) + IAA (0.5 mg/L). AIA 0, 1.0, 2.0 and 3.0 mg/L was applied for rooting and the ex-vitro adaptation of the plants was with peat moss and a mix with peat moss with natural soil (1:1). The best treatment was BAP 3.0 mg/L with 5.13 ± 0.9150 shoots per explant. 89 ± 0.03% of rooted shoots were obtained and the ex-vitro survival was 68.75% in peat moss substrate with natural soil (1:1). A protocol for the clonal propagation of K. grusonii was established, which represents a contribution to its ex-situ conservation.
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