Leukocyte profile as hematologic biomarker in populations of the mountain salamander, Ambystoma ordinarium
DOI:
https://doi.org/10.22201/ib.20078706e.2024.95.5330Keywords:
Disturbed habitats, Immune response, N/L ratio, Band neutrophils, Plasma cells, Achoque michoacanoAbstract
Assessing the leukocyte profile as a hematological biomarker is now frequently used in ecological studies of threatened or declining species. In this study, we evaluated and compared leukocytes profile and neutrophils/lymphocytes (N/L) ratio in peripheral blood smears of the salamander Ambystoma ordinarium in 3 types of habitats: urbanized, agricultural, and conserved. We considered leukocyte profiles as an immunological endpoint, since it can provide information about the immunological response. Results indicated that A. ordinarium individuals from the urbanized and agricultural sites presented higher proportions of neutrophils, eosinophils, basophils and monocytes and a decrease in the proportions of lymphocytes. Agricultural habitats presented higher N/L ratios. Likewise, in the individuals of urbanized and agricultural sites an increase was registered in the number of neutrophils with a band nucleus, in addition, the finding of plasma cells in the blood of this species is reported for the first time. In general, leukocyte profiles of A. ordinarium individuals in urbanized and agricultural sites observed in this study suggest that these profiles can be interpreted as physiological responses to environmental disturbance.
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