Microbiota bacteriana sanguínea de la tortuga texana Gopherus berlandieri en Tamaulipas, México

Blood microbiota of Gopherus berlandieri

Autores/as

  • Eduardo Manolo Medrano-Zapata Tecnológico Nacional de México
  • Jorge Luis Becerra-López Universidad Juárez del Estado de Durango
  • Pedro Almaguer-Sierra Tecnológico Nacional de México
  • Cristina García-De la Peña Universidad Juárez del Estado de Durango https://orcid.org/0000-0003-0937-4466
  • Ludivina Barrientos-Lozano Tecnológico Nacional de México
  • Juan Flores-Gracia Tecnológico Nacional de México
  • Karen Berenice Lara-Rodríguez Tecnológico Nacional de México
  • Felipe Vaca-Paniagua Universidad Nacional Autónoma de México
  • Clara E. Díaz-Velásquez Universidad Nacional Autónoma de México
  • Aldo H. De la Cruz-Montoya Universidad Nacional Autónoma de México

DOI:

https://doi.org/10.22201/ib.20078706e.2024.95.5365

Palabras clave:

Secuenciación, Coxiella, Ehrlichia, Anaplasma phagocytophilum, Salmonella enterica

Resumen

La microbiota bacteriana sanguínea de la tortuga texana (Gopherus berlandieri) en Tamaulipas, México, fue caracterizada mediante secuenciación de siguiente generación. En 2019 se colectó sangre de 6 tortugas silvestres. Se extrajo el DNA, se amplificó la región V3-V4 del gen 16S rRNA y se realizó secuenciación con Illumina. Los resultados mostraron 9 phyla, 20 clases, 42 órdenes, 81 familias, 176 géneros y 299 especies bacterianas. Firmicutes fue el phylum más abundante en la sangre de G. berlandieri; este taxón ha sido registrado como predominante en intestino, excremento, exudados nasales y saliva de otras especies de Gopherus. Los géneros bacterianos dominantes fueron Caldalkalibacillus, Anaerobacillus, Nesterenkonia y Bacillus. Estos taxa se encuentran en suelos alcalinos y halófilos como los que se presentan en las madrigueras de G. berlandieri. Todos estos taxa bacterianos podrían ingresar al torrente sanguíneo vía translocación intestinal, oral y nasal. En la sangre de esta tortuga se registraron 3 taxa (Coxiella sp., Ehrlichia sp. y Anaplasma phagocytophilum) que se trasmiten por artrópodos y el patógeno potencial Salmonella enterica. Esta información es una primera referencia bacteriológica de la sangre de G. berlandieri y se espera que sea de utilidad en programas de salud y conservación.

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2024-05-03

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ECOLOGÍA