Phylogenetic position of Dolops bidentata (Ichthyostraca: Argulidae) based on molecular data: first record of the genus in Mexico



Palabras clave:

Parasitic crustaceans, Branchiura, Argulus, Centropomus undecimalis, COI, 18S rRNA, 28S rRNA


Species included in the genus Dolops (Ichthyostraca: Argulidae) have been recorded from Southern and Central Africa, Australia, and mainly from South America, with no records from Central or North America. Specimens of Dolops bidentata, previously recorded only in South America, were collected in the state of Tabasco, southern Mexico, parasitizing the common snook, Centropomus undecimalis. Here, we provide the first record of the genus and the species in North America (Mexico) and the first molecular characterization of Dolops bidentata including 1 mitochondrial and 2 nuclear DNA markers, as well as a morphological description of the specimens. The newly generated molecular data were used to preliminarily investigate the phylogenetic relationships of Branchiura and to include Dolops bidentata in a phylogenetic hypothesis. Our results fail to recover the monophyly of Dolops; however, more investigations are needed before any taxonomic change is made.

Biografía del autor/a

Omar Lagunas-Calvo, Universidad Nacional Autónoma de México Instituto de Biología

Departamento de Zoología, IB, UNAM

Serapio López-Jiménez, Universidad Juárez Autónoma de Tabasco

Universidad Juárez Autónoma de Tabasco, Villahermosa, Tabasco, México

Alejandro Oceguera-Figueroa, Instituto de Biología, UNAM

Departamento de Zoología, IB, UNAM


Abele, L. G., Kim, W., & Felgenhauer, B. E. (1989). Molecular evidence for inclusion of the phylum Pentastomida in the Crustacea. Molecular Biology and Evolution, 6, 685–691.

Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215, 403–410.

Andres, M. J., Higgs, J. M., Grammer, P. O., & Peterson, M. S. (2019). Argulus from the Pascagoula River, MS, USA, with an emphasis on those of the threatened Gulf Sturgeon, Acipenser oxyrinchus desotoi. Diversity, 11, 232.

Avenant-Oldewage, A., van As, J. G., & Loots, G. C. (1989). On the hatching and morphology of Dolops ranarum larvae (Crustacea: Branchiura). Journal of Zoology, 217, 511–519.

Barton, D. P., & Morgan, J. A. (2016). A morphological and genetic description of pentastomid infective nymphs belonging to the family Sebekidae Sambon, 1922 in fish in Australian waters. Folia Parasitologica, 63, 1.

Bouvier, E. L. (1899a). Les Crustacés parasites du genre Dolops Audouin (Première Partie). Bulletin de la Société Philomathique de Paris, 8, 53–81.

Bouvier, E. L. (1899b). Les Crustacés parasites du genre Dolops Audouin (Seconde Partie). Bulletin de la Société Philomathique de Paris, 9, 12–40.

Costa, F. O., DeWaard, J. R., Boutillier, J., Ratnasingham, S., Dooh, R. T., Hajibabaei, M. et al. (2007). Biological identifications through DNA barcodes: the case of the Crustacea. Canadian Journal of Fisheries and Aquatic Sciences, 64, 272–295.

Darriba, D., Taboada, G. L., Doallo, R, & Posada, D. (2012). jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9, 772.

Feroz-Khan, K., Sanker, G., & Prasanna-Kumar, C. (2014). Linking eggs and adults of Argulus spp. using mitochondrial DNA barcodes. Mitochondrial DNA Part A, 27, 3927–3931.

Fontana, M., Takemoto, R. M., Malta, J. C. O., & Mateus, L. A. F. (2012). Parasitism by argulids (Crustacea: Branchiura) in piranhas (Osteichthyes: Serrasalmidae) captured in the Caiçara bays, upper Paraguay River, Pantanal, Mato Grosso state, Brazil. Neotropical Ichthyology, 10, 653–659.

Fryer, G. (1964). Further studies on the parasitic Crustacea of the African freshwater fishes. Proceedings of the Zoological Society of London, 143, 79–102.

Fryer, G. (1968). The parasitic Crustacea of African freshwater fishes; their biology and distribution. Journal of Zoology, 156, 45–95.

Hovmöller, R., Pape, T., & Källersjö, M. (2002). The Palaeoptera problem: basal pterygote phylogeny inferred from 18S and 28S rDNA sequences. Cladistics, 18, 313–323.

Katoh, K., Rozewicki, J., & Yamada, K. D. (2019). MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics, 20, 1160–1166.

Lagunas-Calvo, O., García-Prieto, L., Osorio-Sarabia, D., LeónRègagnon, V., & Oceguera-Figueroa, A. (2020). New records of Ichthyostraca Zrzavý, Hypša & Vlášková, 1997 (Pancrustacea) from Mexico with an annotated checklist of North America. Zootaxa, 4775, 1–55.

Lanfear, R., Calcott, B., Ho, S. Y., & Guindon, S. (2012). PartitionFinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology and Evolution, 29, 1695–1701.

Lavrov, D. V., Brown, W. M., & Boore, J. L. (2004). Phylogenetic position of the Pentastomida and (pan) crustacean relationships. Proceedings of the Royal Society of London. Series B: Biological Sciences, 271, 537–544.

Maddison, W. P., & D. R. Maddison. (2007). Mesquite: a modular system for evolutionary analysis. Version 2.0

Malta, J. C. D. O. (1982). Os argulídeos (Crustacea: Branchiura) da Amazônia Brasileira. Aspectos da ecologia de Dolops discoidalis Bouvier, 1399 e Dolops bidentata Bouvier, 1899. Acta Amazonica, 12, 521–528.

Malta, J. C. D. O. (1984). Os peixes de um lago de Várzea da Amazônia central (Lago Januacá, Rio Solimões) e suas relações com os crustáceos ectoparsitas (Branchiura: Argulidae). Acta Amazonica, 14, 355–372.

Messing, J. (1993). M13 cloning vehicles: their contribution to DNA sequencing. Methods in Molecular Biology, 23, 9–22.

Møller, O. S., & Olesen, J. (2010). The little-known Dipteropeltis hirundo Calman, 1912 (Crustacea, Branchiura): SEM investigations of paratype material in light of recent phylogenetic analyses. Experimental Parasitology, 125, 30–41.

Møller, O. S., & Olesen, J. (2012). First description of larval stage 1 from a non-African fish parasite Dolops (Branchiura). Journal of Crustacean Biology, 32, 231–238.

Møller, O. S., Olesen, J., Avenant-Oldewage, A., Thomsen, P. F., & Glenner, H. (2008). First maxillae suction discs in Branchiura (Crustacea): development and evolution in light of the first molecular phylogeny of Branchiura, Pentastomida, and other “Maxillopoda”. Arthropod Structure and Development, 37, 333–346. asd.2007.12.002

Mwita, C. J., & Nkwengulila, G. (2010). Phylogenetic relationships of the metazoan parasites of the clariid fishes of Lake Victoria inferred from partial 18S rDNA sequences. Tanzania Journal of Science, 36, 47–57.

Neethling, L. A., & Avenant-Oldewage, A. (2016). Branchiura —a compendium of the geographical distribution and a summary of their biology. Crustaceana, 89, 1243–1446.

Patra, A., Mondal, A., Banerjee, S., Adikesavalu, H., Joardar, S. N., & Abraham, T. J. (2016). Molecular characterization of Argulus bengalensis and Argulus siamensis (Crustacea: Argulidae) infecting the cultured carps in West Bengal, India using 18S rRNA gene sequences. Molecular Biology Research Communications, 5, 156–166.

Patterson, C. (1996). Comments on Mabee's “Empirical rejection of the ontogenetic polarity criterion”. Cladistics, 12, 147– 168.

Pearse, A. S. M. (1920). The fishes of Lake Valencia, Venezuela. University of Wisconsin Studies in Science, 12, 1–51.

Phillips, A. J., Arauco-Brown, R., Oceguera-Figueroa, A., Gomez, G. P., Beltrán, M., Lai, Y. T. et al. (2010). Tyrannobdella rex n. gen. n. sp. and the evolutionary origins of mucosal leech infestations. Plos One, 5, e10057.

Poly, W. J. (2008). Global diversity of fishlice (Crustacea: Branchiura: Argulidae) in freshwater. Hydrobiologia, 595, 209–212.

Prosser, S. W. J., Velarde-Aguilar, M. G., León-Règagnon, V., & Hebert, P. D. N. (2013). Advancing nematode barcoding: A primer cocktail for the cytochrome oxidase subunit I gene from vertebrate parasitic nematodes. Molecular Ecology Resources, 13, 1108–1115.

Rambaut, A. (2012). FigTree v1.4. Available from

Ringuelet, R. A. (1948). Argulídos del Museo de la Plata. Revista del Museo de La Plata, 5, 281–296.

Siddall, M. E., & Whiting, M. F. (1999). Long-branch abstractions. Cladistics, 15, 9–24.

Silva-Souza, A. T., Abdallah, V. D., De Azevedo, R. K., Da Silva, F. A., & Luque, J. L. (2011). Expanded description of Dolops bidentata (Bouvier, 1899) (Branchiura: Argulidae) based on specimens collected on Pygocentrus nattereri Kner, 1858 (Characiformes) from Poconé Wetland, MT, Brazil. Brazilian Journal of Biology, 71, 145–149.

Silvestro, D., & Michalak, I. (2011). RaxmlGUI: A graphical front-end for RAxML. Organisms Diversity and Evolution, 12, 335–337.

Tautz, D., Hancock, J. M., Webb, D. A., Tautz, C., & Dover, G. A. (1988). Complete sequences of the rRNA genes of Drosophila melanogaster. Molecular Biology and Evolution, 5, 366–376. a040500

Wadeh, H., Alsarakibi, M., & Li, G. (2010). Analysis of genetic variability within Argulus japonicus from representatives of Africa, Middle East, and Asia revealed by sequences of three mitochondrial DNA genes. Parasitology Research, 107, 547–553.

Whiting, M. F. (2002). Mecoptera is paraphyletic: multiple genes and phylogeny of Mecoptera and Siphonaptera. Zoological Scripta, 31, 93–104.

Wilson, C. B. (1902). North American parasitic copepods of the family Argulidae, with a bibliography of the group and a systematic review of all known species. Proceedings of the United States National Museum, 25, 635–742.

Woodyard, E. T., Stilwell, J. M., Camus, A. C., & Rosser, T. G. (2019). Molecular and histopathological data on Levisunguis subaequalis Curran, Overstreet, Collins and Benz, 2014 (Pentastomida: Eupentastomida: Porocephalida: Porocephaloidea: Sebekidae: Sebekinae) from Gambusia affinis in Alabama, USA. Journal of Parasitology, 105, 827–839.

Zrzavý, J., Hypša, V., & Vlášková, M. (1997). Arthropod phylogeny: taxonomic congruence, total evidence and conditional combination approaches to morphological and molecular data sets. In R. A. Fortey, & R. H. Thomas, (Eds.), Systematics Association, Special Volume Series 55. London: Chapman and Hall.