Genetic connectivity of the endangered brown sea cucumber Isostichopus fuscus in the northern Gulf of California revealed by novel microsatellite markers

Autores/as

  • José Miguel Ochoa-Chávez Centro de Investigación Científica y de Educación Superior de Ensenada
  • Miguel Ángel Del Río-Portilla Centro de Investigación Científica y de Educación Superior de Ensenada
  • Luis Eduardo Calderón-Aguilera Centro de Investigación Científica y de Educación Superior de Ensenada
  • Axayácatl Rocha-Olivares Biological Oceanography Department > CICESE http://orcid.org/0000-0002-2700-9086

DOI:

https://doi.org/10.22201/ib.20078706e.2018.2.2294

Palabras clave:

Holothurid, Fisheries, Conservation genetics, Next generation sequencing, Genome sequencing

Resumen

Isostichopus fuscus is an economically important sea cucumber that has been highly exploited along its distribution range in the eastern Pacific. The significant population decline is responsible for its listing as endangered in the IUCN Red List. Despite its importance for management and conservation, information about its population genetic structure
is unavailable, largely due to the lack of suitable genetic markers. Here we develop species-specific microsatellite markers and use them to assess the genetic connectivity between populations in the Gulf of California. Next generation sequencing (Illumina) was used to shotgun-sequence the genome of 2 sea cucumbers. From these data, we identified
and characterized 19 polymorphic microsatellite loci; which were tested in organisms from Bahía de los Ángeles, on the western shore of the Gulf of California. The number of alleles ranged from 5 to 22, observed heterozygosity from 0.35 to 1, and 4 loci deviated from Hardy-Weinberg equilibrium. We determined high levels of genetic connectivity between this locality and San Felipe, in the upper gulf (Amova φst = 0.002; p > 0.05) with a subset of 8 markers. The newly designed microsatellites are suitable for multiplexing panels and will be useful for the future genetic assessment of this important tropical sea cucumber.

Biografía del autor/a

José Miguel Ochoa-Chávez, Centro de Investigación Científica y de Educación Superior de Ensenada

Departamento de Oceanografía Biológica

Miguel Ángel Del Río-Portilla, Centro de Investigación Científica y de Educación Superior de Ensenada

Departamento de Acuicultura

Luis Eduardo Calderón-Aguilera, Centro de Investigación Científica y de Educación Superior de Ensenada

Departamento de Ecología Marina

Axayácatl Rocha-Olivares, Biological Oceanography Department > CICESE

Departamento de Oceanografía Biológica

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Publicado

2018-06-04

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CONSERVACIÓN